KCNMA1 and Anxiety: Intriguingly, BK channel activation also principally negatively affects pain signalling at supraspinal levels such as the anterior cingulate cortex, the motivational–affective centre and a key hub for anxiety and pain perception, although overexpression of the synaptic and non-synaptic BK channel β4 subunit evoked mechanical allodynia along with anxiety-like behaviour [213].