TWIST2 and congenital myasthenic syndrome: In this study, transcriptome analysis showed that the genes involved in tapetal and microspore development, such as PG, pectinesterase, pollen coat protein, callose synthase, GDSL esterase/lipase and cellulose synthase A subunit, AMS, bHLH089, and bHLH091, were significantly down-regulated in the Ogura CMS line.