Importantly, we found that different cancer cell lines, such as A549, U87, Hep3B, PANC-1, and HCT116, expressed high level of caspase 3 but not MCF7, and the cleaved caspase 3 was notably enhanced in A549, U87, PANC-1, and HCT 116 cells after treatment with compound 6d, while it was not cleaved in Hep3B cells (Figure 5F). The gene discussed is CASP3; the disease is cancer.