An Annexin V-FITC/PI assay was employed via flow cytometry after CRC cells exposure to compound 6d for 8 h, and typical images and histograms in Figure 2A showed that compound 6d significantly induced cellular apoptosis in all CRC cells and the proportion of late-phase apoptosis was dramatically increased in a dose-dependent manner (from 2.85% to 66.1% for HCT 116 cells; from 4.47% to 25.8% for LN229 cells; from 4.63% to 52.7% for SW480 cells). This evidence concerns the gene ANXA5 and colorectal carcinoma.