TNF and infection: In the case of invasion assay, the results of HDGF and TNFα were variable depending on infection with different strains of H. pylori. In this assay, after one week of incubation, the organoids were dissociated with TrypLETM Express Enzyme (Thermo Fisher Scientific, Waltgam, MA, USA) and cells were transferred into Transwell plates with the Matrigel on the surface, for cultivation for 6 days and then the growth and organoid invasion area were evaluated.