From the list of genes that showed relatively increased expression in metastatic cells, immunohistochemistry targeted to EGFR, MMP2, IL1B and MAGEC1 epitope expression showed strong positive cytoplasmic staining in the tumor cells of LNM and BM patient samples (Positive or High Positive score), while melanoma cells at the primary site had a low expression of these markers (Low Positive), results which were corroborated at gene expression level (more than 20-fold change). The gene discussed is IL1B; the disease is melanoma.