To determine the effect of Cad-11 in cardiac hypertrophy via MAPK and CaMKII-STAT3 pathways in vivo, we performed TAC in Cad-11−/− and WT mice and MAPK and CaMKII activation was measured by Western blotting using heart samples from those mice (Figure 8A–D). This evidence concerns the gene STAT3 and cardiac hypertrophy.