KCND2 and persistent truncus arteriosus: Further, to determine whether Cad-11 deletion affects cardiac repolarization ion channel gene expression levels under TAC treatment, we employed qRT-PCR to measure the mRNA levels of ion channel genes Cav1.2 (for ICa++), Kv4.2/Kv4.3/KCHIP2 (for Itof), and Kv1.5/Kv2.1 (for Ikslow) genes.