TIAM1 and hepatocellular carcinoma: Consistent with this hypothesis, the methylation pattern across TIAM1 in the subgroup-2 HCC cell lines was highly correlated with methylation in the normal HHL5 cell line (r2 = 0.75, p = 0.0005) and methylation levels across the differential methylated region (DMR) were very similar (average methylation across the TIAM1 DMR was 16% vs. 15% vs. 62% in HHL5, subgroup-2 HCC cell lines, and non-subgroup-2 HCC cell lines, respectively, Supplemental Table S1).