To further evaluate the role of the ectopic overexpression of ARv7 in the growth of prostate cancer cells, we used the EdU proliferation assay to determine the growth of PC-DNA and PC-ARv7 cells and the Ki67 proliferation assay to assess the growth of 22Rv1-DNA and 22Rv1-ARv7 cells. This evidence concerns the gene MKI67 and prostate cancer.