The strengths of the study are (i) exclusively treatment-naïve GBM grade IV patients, (ii) with inclusion of MGMT protein expression with the evaluation of the nucleus and cytoplasmic distribution, the cytoplasmic localization could indicate the trafficking of MGMT loaded with methyl towards proteasome for degradation, and (iii) balanced groups for age, MGMT promoter methylation status and surgical resection type. The gene discussed is MGMT; the disease is glioblastoma.