In order to investigate a possible role of PATZ1 overexpression in downregulating PD-L1 expression in NSCLC cells, we transiently transfected A549 and H1299 cells with a plasmid expressing the human PATZ1 gene and analyzed PATZ1 and PD-L1 protein levels 24 h later by Western blot (Figure 5). This evidence concerns the gene CD274 and non-small cell lung carcinoma.