CDC6, DHFR, E2F1, H2AFZ, MCM2, GUSB and B2M were significantly higher in patients with metastatic disease than in healthy controls, and CDC6, DHFR and E2F1 were significantly higher in metastatic patients than in localized patients (Figure 2 and Supplemental Table S9). Here, DHFR is linked to metastatic neoplasm.