HBE1 and anemia (phenotype): Taken together, the findings of the present study revealed that the codon 26 (GAG>AAG) HbE (βE) mutation; (β0)-thalassaemia Filipino ~45 kb deletion; IVS 1-2 (T>C) (β0) with 26 (GAG>AAG) HbE (βE); Cd 41/42 (-TTCT), IVS 1-5 (G>C) (β+) with Cd 26 (G>A); and Cd 41/42 (-TTCT) (β0), IVS 1-5 (G>C) (β+), and Cd 17 (A>T) mutations of HbE/β-thalassaemia patients are associated with severe anaemia and iron overload compared to other mutations, suggesting that the severity of the disease could be attributed to these mutations.