Taken together, the findings of the present study revealed that the codon 26 (GAG>AAG) HbE (βE) mutation; (β0)-thalassaemia Filipino ~45 kb deletion; IVS 1-2 (T>C) (β0) with 26 (GAG>AAG) HbE (βE); Cd 41/42 (-TTCT), IVS 1-5 (G>C) (β+) with Cd 26 (G>A); and Cd 41/42 (-TTCT) (β0), IVS 1-5 (G>C) (β+), and Cd 17 (A>T) mutations of HbE/β-thalassaemia patients are associated with severe anaemia and iron overload compared to other mutations, suggesting that the severity of the disease could be attributed to these mutations. Here, HBE1 is linked to Tangier disease.