We found that tumor-specific CD8+ TILs produced more IFNγ; blood-derived tumor-specific CD8+ T cells produced more TNF and IL-2 after restimulation with PMA and ionomycin (Fig. 7d, e and Supplementary Fig. 13d, e) and contained more poly-functional (IFNγ/TNF/IL-2-coexpressing) cells (Fig. 7e and Supplementary Fig. 13e). This evidence concerns the gene TNF and neoplasm.