SIM2 bound to SUMO1 in vitro (Supplementary Fig. S12b) and blocked de novo lipogenesis in various types of cancer cells, including breast (MDA-MB-231, MDA-MB-468 and Hs578T), prostate (R1-AD1 and R1-D567), colon (HCT116), and mouse tumor cells (4T1 and CT26.CL25) (Supplementary Fig. S12c–h). The gene discussed is SUMO1; the disease is cancer.