GBA1 and neuroblastoma: To this end, we used a variety of GCase-deficient cell lines: immortalised mouse cortical neurons from embryonic null allele Gba−/− mice26, human pluripotent stem cell-derived neurons or human neuroblastoma cells (H4 cells) in which GBA1 was deleted (GBA−/−)27 or primary murine neurons harbouring a human Gba1 homozygous mutation (Gba D409V/D409V)28.