Though ΔEhaF infection induced higher cell death at 18 h post-infection (Supplementary Fig. 1o–s), increased induction of IFNβ and IL-6 responses by ΔEhaF was not due to increased pyroptosis as IFNβ and IL-6 production is an early response assessed at 2–6 h post-infection whereas pyroptosis begins to occur only after 8 h and reaches higher levels at 18 h (Supplementary Fig. 1o–s). This evidence concerns the gene IFNB1 and infection.