In addition, stable knockingdown or overexpression of PTBP2 led to an decrease or increase in nuclear levels of IRF9, STAT1, and STAT2 but slightly affected the cytoplasmic levels of IRF9 and STAT1 in NB cells (Fig. 5C), indicating that PTBP2 alteration has a positive correlation with IRF9 and STAT1 expression, as well as ISGF3 formation. This evidence concerns the gene IRF9 and neuroblastoma.