In view of prior documentation of low podocyte ZHX2 expression in human MCD and FSGS (27), the insertion at Chr8: 122,533,694 was replicated in a single cell–derived cultured human podocyte cell line using CRISPR/Cas9 technology (study CRISPR B, Figure 6C). This evidence concerns the gene ZHX2 and focal segmental glomerulosclerosis.