The frequency of tetramer+ cells was variable in granulomas as well as within and across animals, with a median frequency of 0.83% of CD4 T cells staining positive for CFP-10 tetramers and a median frequency of 1.27% of CD4 T cells staining positive for the Rv1196371-385 tetramer; in contrast, the median frequency of Rv012581-92 tetramer+ CD4 T cells was relatively low at 0.20% (Fig. 4B–E) (example flow cytometry plots in Fig. S2, , –, S5). Here, CD4 is linked to Granuloma.