In the spleen, we found that Gpr83+Igfbp4+ naive Treg cells (P6 and S6) newly appeared in mice after infection, with an increased number of cells from the Lag3hi subpopulation (P0 and S0), the Tconv population (P2 and S2), cluster 3 (P3 and S3), CD25hi naive Treg cells (P4 and S4), and the Helioshi subpopulation (P5 and S5) and a decreased quantity of cells from the Foxp3hi subpopulation (P1 and S1). This evidence concerns the gene IGFBP4 and infection.