By contrast, Unc-5 knockdown had no effect on the RE phenotype caused by the expression of ALS-associated FUS alleles that cause severe degenerative phenotypes in Drosophila (Figure 3—figure supplement 1; Lanson et al., 2011; Kwiatkowski et al., 2009; Vance et al., 2009). This evidence concerns the gene FUS and amyotrophic lateral sclerosis.