To gain further insight into the neutralization spectra of DNA vaccine-generated NAbs, we next compared MAP-1002 and pWT/IM + EP antisera for ability to block mimic infection of hACE2-transgenic HEK293T cells by a set of pseudoviruses displaying the S protein of WT SARS-CoV-2 or variant Beta, Delta, or Omicron subvariant BA.1, BA.2 or BA.4/5. This evidence concerns the gene PROS1 and infection.