PTK2 and acute myeloid leukemia: Western blotting was performed to detect the phosphorylation level of the key signal transducers in the common pathways involved in the development of malignancy; the phosphorylation of FAK, PI3K, Akt, and Nuclear Factor- Kappa B (NF-κB) was significantly elevated in AML cells treated with 50 ng/mL netrin-1 for 6 h (Figure 4a).