PCLO and neoplasm: The maximum VAF of somatic mutations of genes (AHNAK2, TTN, MUC17, MUC16, MAGEC1, FAM47C, MACF1, RPL1, FLG, PCLO, and ZNF208) in tumor tissues was positively correlated with that in cfDNA (Cor = 0.759; R2 = 0.576; p = 2.65 × 10–21, Fig. 6B).