Furthermore, ZDHHC3 expression was mutually exclusive of four immune checkpoints, including PD-1, cytotoxic T lymphocyte antigen 4 (CTLA-4), and lymphocyte activation gene 3 (LAG-3) expression in some cancer types, including PAAD (Fig. 3D–G). This evidence concerns the gene ZDHHC3 and pancreatic adenocarcinoma.