Meanwhile, compared with normal hepatocytes (CCC-HEL-1), the mRNA and protein levels of RNF2 were significantly elevated in six HCC cell lines (Hep-G2, BEL-7402, Huh-7, Hep-3B, MHCC-97H, MHCC-LM3) (Fig. 1G, H; Supplementary Fig. S9). This evidence concerns the gene RNF2 and hepatocellular carcinoma.