OPN1SW and infection: The investigators then used recombinant HBV constructs to study different combinations of the PC/BCP mutations compared to wildtype HBV (HBV-wt/wt, HBV-mt/mt, HBV-wt/mt, HBV-mt/wt) and demonstrate that in their mouse model, infection with the double-mutant and both single-mutant constructs led to higher viral loads followed by a decrease of hepatocyte viability compared to infection with wildtype.