The most common signs of fibrosis in NASH are mainly caused by excessive consumption of high-fat components, where patients absorb nutrients The HFD-induced liver fibrosis model overcomes the shortcomings of the MCD-induced liver fibrosis model, in which animals with increased body weight and peripheral insulin resistance develop and mimic the etiology of the disease by replicating poor dietary habits, with phenotypic features similar to those of human nonalcoholic steatohepatitis. This evidence concerns the gene INS and metabolic dysfunction-associated steatohepatitis.