Consistent with results indicating a role for EZH2 in blocking differentiation in an MLL-AF9 driven mouse model of AML (Tanaka et al., 2012), EZH2 KD induced differentiation in HL-60 cells as measured by flow cytometric analysis of the percentages of cells expressing CD11b (CD11b positive, CD11b+ cells) in either the absence or presence of ATRA (ranging between 2.44-fold and 3-fold, Figure 1A, left panel; Supplementary Figure S1). The gene discussed is EZH2; the disease is acute myeloid leukemia.