In order to assess the impact of EZH2 expression on myeloid differentiation of human non-APL AML, we first examined the effect of EZH2 knockdown (KD) via lentiviral transduction of two short hairpin sequences (shEZH2#1 and shEZH2#2) targeting separate sequences on EZH2 mRNA in MYC-amplified human HL-60 cells (AML FAB M2) (Collins et al., 1977), a long-established model system in this context (Birnie, 1988). Here, EZH2 is linked to acute promyelocytic leukemia.