In Matrigel invasion studies, fibroblast-derived CXCL12 increased the invasiveness of GEM-R PaCa cells treated with GEM, while AMD11070 and KRH3955 significantly decreased it.GEM enhanced the tumorigenicity of GEM-R PaCa cells in vivo, whereas the addition of CXCR4 antagonists dramatically reduced it. The gene discussed is CXCL12; the disease is permanent neonatal diabetes mellitus-pancreatic and cerebellar agenesis syndrome.