To test this hypothesis, cardiomyocyte-specific TLR4 knockdown (cTLR4KO) mice were generated by crossing floxed TLR4 mice with Myh6-Cre mice, and subjected to angiotensin II (Ang II, 1 μg/kg/min or vehicle for 14 days) hypertension model. Here, TLR4 is linked to hypertensive disorder.