CD4 and neoplasm: A second method, using a stringent standard that labeled as memory anti-tumor only clonotypes that reached a higher frequency following stimulation than any control values, found that unique clonotypes varied from 0-18 CD8 and 0-5 CD4 T-cell TRA or TRB anti-tumor clonotypes per animal (median CD8 = 2.5 and CD4 = 1.5).