IL4 and cancer: However, it should be emphasized that even when restimulated spleen cells produced high amount of IL-4, IL-10 together with increased concentration of IFN-γ, the effect caused by HES-MTX + DC/shIL-10R/TAg therapy at the local anti-tumor immune response level, was strong enough to elicit the antigen-specific systemic immune response manifested by the greatest ability of splenocytes to eliminate cancer cells.