Similar to CD8+ T cells subpopulation, the treatment with nanoconjugate and DC/shIL-10R/TAg caused a significantly increased influx of CD4+ T cells into tumor tissue, not only in comparison to MTX + DC/shIL-10R/TAg group, but also in relation to other HES-MTX-treated groups of mice. Here, CD4 is linked to neoplasm.