For further investigation, we examined histological analysis for testes in the CYP-treated group and discovered that CYP-induced testicular impotence in the form of azoospermia and seminiferous tubule atrophy caused tissue injury due to its cytotoxicity by generating ROS that affected the architecture of the testes tissue and caused damage to Sertoli cells, Leydig cells, and spermatozoa, resulting in no sperms, resulting in infertility (Namasivayam et al., 2006; Comish et al., 2014; Zhu et al., 2015; Hosseini et al., 2018; Merwid-Ląd et al., 2021). Here, PPIG is linked to Azoospermia.