NFKB1 and cardiac hypertrophy: To clarify the mechanism by which cardiac hypertrophy did not occur in Ogt-Tg mice after TAC4W, Western blot and immunoprecipitation analyses were performed to examine the activities of NF-κB and GSK-3β, which are major regulators of cardiac hypertrophy and O-GlcNAcylated proteins (21, 32).