In our study, we found that LY dramatically down-regulated the protein and mRNA expressions of TLR-4, NF-κB, NLRP3, Caspase-1, and IL-1β in the livers of NAFLD mice, indicating the inhibition of the NLRP3 inflammasome related pathway. This evidence concerns the gene TLR4 and metabolic dysfunction-associated steatotic liver disease.