THRAP3 and malignant colon neoplasm: To disclose the functions of THRAP3S248p and THRAP3S253p in colon cancer, we silenced the THRAP3 gene in the HCT116 cell line (Fig. 7A) and then overexpressed two pairs of THRAP3 mutants, Flag-THRAP3S248A and Flag-THRAP3S248D, as well as Flag-THRAP3S253A and Flag-THRAP3S253D, in the THRAP3-KD HCT116 cell line (Fig. 7B).