Human NAFLD tissue homozygous for the 446 L allele (rs1260326,TT) had lower GKRP and GK protein levels and lower ratios of nuclear GK/nuclear GKRP, which concurs with the lower expressivity of the GKRP-446 L variant and the lower nuclear GK sequestration. This evidence concerns the gene GCKR and metabolic dysfunction-associated steatotic liver disease.