GCKR and hepatocellular carcinoma: When GKRP (446 P or 446 L) was expressed in heterologous cell lines (FTO2B hepatoma and HeLa cells) which lack endogenous GK, and without or with adenoviral-mediated GK expression, the sequestration of GKRP in the nucleus was increased ∼2 fold by GK expression (Figure.