When we tested an in vitro assay for cell invasive migration, that mimics a part of metastasis (see Materials and Methods), using two human colon cancer cell lines (DLD1 and HCT116) overexpressing FRNK, the expression of GFP-FRNK, which was confirmed to facilitate cell adhesion in both cell lines, activated invasive migration in the DLD1 cell line (Supplementary Fig. S10A, B). This evidence concerns the gene PTK2 and malignant colon neoplasm.