PTK2 and colonic neoplasm: When we tested an in vitro assay for cell invasive migration, that mimics a part of metastasis (see Materials and Methods), using two human colon cancer cell lines (DLD1 and HCT116) overexpressing FRNK, the expression of GFP-FRNK, which was confirmed to facilitate cell adhesion in both cell lines, activated invasive migration in the DLD1 cell line (Supplementary Fig. S10A, B).