PRPF19 and colorectal carcinoma: Based on the fact that the basal expression of PRPF19 was the lowest in DLD1 cells, and the highest in HCT116 cells among the seven CRC cell lines (Supplementary Fig. 1C), HCT116, DLD1 were selected to construct the stable cell lines with PRPF19 knockdown (scramble/shPRPF19-1/shPRPF19-2) or overexpression (Ctrl/PRPF19), and the efficiency was validated by Western blot (Fig. 2A, Supplementary Fig. 3A).