After systematic administration, NPTyr-C9AP with Tyr promoter were demonstrated to induce specific coexpression of CXCL9 and αPD-L1 in multiple melanoma models, while NPSur-C9AP with Sur promoter specifically coexpressed CXCL9 and αPD-L1 in different tumors. Here, CXCL9 is linked to melanoma.