In addition, as shown in Fig. 2j, k, the concentrations of CXCL9 and αPD-L1 in melanoma of NPTyr-C9AP-treated mice were significantly increased to 12.12 and 43.12 ng per gram (ng g−1) tumor tissue; as expected, the concentrations of CXCL9 and αPD-L1 in the pancreatic tumor, heart, liver, spleen, lung or kidney were not increased when compared to that of NPContorl group. This evidence concerns the gene CXCL9 and melanoma.