B16-F10 melanoma-bearing mice were intravenously injected with PBS, anti-PD-L1 antibody, NPTyr-αPD-L1, NPTyr-CXCL9 & anti-PD-L1 antibody or NPTyr-C9AP every other day for five injections (Fig. 6a). This evidence concerns the gene CXCL9 and melanoma.