As assessing radiosensitivity in advanced-stage laryngeal cancer may have a significant impact on treatment policy in advanced stage laryngeal cancer, the aim of this study was to assess the prognostic value of tumour markers for hypoxia (HIF-1α and CA-IX) and proliferation (Ki-67) in a well-defined consecutive series of patients with advanced stage (T3–T4, M0) laryngeal squamous cell carcinoma treated with primary, curatively intended radiotherapy with regards to locoregional recurrent disease and disease-specific mortality. This evidence concerns the gene MKI67 and neoplasm.