Most of the previous analyses were based on the bulk RNA sequencing of the tumor tissue; however, the tissue intermixed with numerous cell heterogeneity, such as the tumor, inflammatory cells, endothelial cells, etc. To investigate whether tumor heterogeneity impacts the AUP1 expression, we analyzed two single-cell sequencing databases, GSE131928 and GSE89567, representing IDH wildtype and IDH mutant astrocytoma, respectively. The gene discussed is AUP1; the disease is astrocytoma (excluding glioblastoma).