Other significant genetic variants such as COL22A1 (rs62522074, P = 1.39 × 10− 8, adjusted P = 1.54 × 10− 6) DIP2C (rs61831032, P = 1.06 × 10− 8, adjusted P = 1.27 × 10− 6) were observed by GWEIS for anxiety; LRRFIP1 (rs62194228, P = 6.02 × 10− 10; rs3806505, P = 7.57 × 10− 10; rs55874185, P = 7.62 × 10− 10) and ANPEP (rs41276918, P = 1.31 × 10− 8) were observed by GWEIS for self-harm, but limited epidemiological and biological evidence illustrated their potential effects. The gene discussed is ANPEP; the disease is Anxiety.