Representative flow plots of CD4+CD25+ T cells and Tregs subsets of ITP patients with V216I mutation, T340M mutation and WT were shown in Fig. 1A. The frequency of total CD4+CD25+ T cells and rTregs in the CD4+ T cells was significantly elevated in ITP-V216I/T340M group (p = 0.012 and p < 0.001, respectively, Fig. 1B). This evidence concerns the gene CD4 and autoimmune thrombocytopenic purpura.