Specifically, to screen for functional BiTEs based on T cell activation, we employed a T-cell reporter system (“Jurkat-ZsG”) derived from Jurkat cell (E6.1) that expresses ZsGreen fluorescent protein downstream of TCR activation pathway driven by a promoter with 6× nuclear factor of activated T-cell (NFAT) transcriptionally responsive elements (TREs)60 to interrogate BiTE mediated T-cell/tumor cell interaction and T-cell activation (Fig. 3a). Here, PATZ1 is linked to neoplasm.