Further study validated that TSLC1 down regulated the transcriptional activity of Tcf4/β catenin and inhibited the expression of CyclinD1 and C-myc, thereby killing Wnt abnormally activated liver cancer cells, which were further confirmed in the mouse xenograft tumor model of human hepatocellular carcinoma SMMC-7721.365 In the genome of AdV, 922–947 bp of E1A is the binding region of RB family. The gene discussed is RB1; the disease is neoplasm.