Targeting the Wnt signaling pathway, recombinant adenovirus Ad.wnt-E1A(Δ24bp)-TSLC1 expressing lung cancer suppressor-1 was constructed.364 Ad.wnt-E1A(Δ24bp)-TSLC1 could target and kill cells with abnormal activated Wnt signaling pathway, while showing no obvious killing effect on normal cells. This evidence concerns the gene DHTKD1 and lung cancer.