CLK1 inhibition by 10 μM concentration of Leucettine L41 (inhibit both DYRKs and CLKs) treatment was shown to increase LC3 foci formation and induce autophagy in a dose-dependent manner in human osteosarcoma cells U-2 OS.100 Meanwhile, CLK1 RNA interference in U-2 OS cells resulted in elevated cell autophagy. Here, CLK1 is linked to osteosarcoma.