KEGG pathway annotation showed that the significantly up-regulated pathways mainly involved circadian entrainment, renin secretion, vascular smooth muscle contraction, transcriptional misregulation in cancer, etc.; the significantly down-regulated pathways included osteoclast differentiation, B cell receptor signaling pathway, cytokine receptor interaction, signaling pathways regulating pluripotency of stem cells, hippo and wnt signaling pathway, etc (Fig 3D, S4 File). The gene discussed is REN; the disease is cancer.