The role of the cholinergic system in male sexual health has been reviewed and described by [42] in the handbook of clinical neurology In the present study, an increase in blood AChE level and pro-inflammatory cytokines; IL-1β, IL-6, and TNF-α were observed, suggesting the involvement of cholinergic system and low-grade systemic inflammation in infertility condition. The gene discussed is ACHE; the disease is Infertility.